Purification and characterization of seven bioactive compounds from the newly isolated Streptomyces cavourensis TN638 strain via solid-state fermentation.
Identifieur interne : 000654 ( Main/Exploration ); précédent : 000653; suivant : 000655Purification and characterization of seven bioactive compounds from the newly isolated Streptomyces cavourensis TN638 strain via solid-state fermentation.
Auteurs : Fatma Kaaniche [Tunisie] ; Abdelaaty Hamed [Égypte] ; Lobna Elleuch [Tunisie] ; Ahlem Chakchouk-Mtibaa [Tunisie] ; Slim Smaoui [Tunisie] ; Ines Karray-Rebai [Tunisie] ; Imed Koubaa [Tunisie] ; Guillaume Arcile [France] ; Noureddine Allouche [Tunisie] ; Lotfi Mellouli [Tunisie]Source :
- Microbial pathogenesis [ 1096-1208 ] ; 2020.
Abstract
The strain TN638 was isolated from Tunisian soil contaminated with industrial wastewater and selected for its potent antimicrobial activity against the tested Gram positive bacteria: Staphylococcus aureus (S. aureus) ATCC 6538 and Listeria monocytogenes (L. monocytogenes) ATCCC 19117, and Gram negative bacteria: Agrobacterium tumefaciens (A. tumefaciens) ATCC 23308 and Salmonella typhimurium (S. typhimurium) ATCC 14028 and fungi: Candida albicans (C. albicans) ATCC 10231, Rhizoctonia solani (R. solani) ATCC 58938 and Fusarium sp. Solide-state fermentation (SSF) dry crude extract of the TN638 strain presents a strong inhibitory activity notably against the phytopathogenic microorganism A. tumefaciens ATCC 23308 and the two pathogenic bacteria S. aureus ATCC 6538 and L. monocytogenes ATCCC 19117 with a zone of inhibition of 48, 34 and 34 mm respectively. According to the morphological characteristic, the complete 16S rRNA gene nucleotide sequence determination [1492 bp deposited in National Center of Biotechnology Information (NCBI) database under the accession no. LN854629.1; https://www.ncbi.nlm.nih.gov/nuccore/LN854629.1/], and the phylogenetic analysis, we can deduce that our isolate is an actinomycete bacterium belonging to the genus Streptomyces and the most closely related strain was Streptomyces cavourensis (S. cavourensis) NRRL 2740T (99.9%). We propose the assignment of our strain as Streptomyces cavourensis (S. cavourensis) TN638 strain. Work-up and purification of the strain extract using different chromatographic techniques afforded seven bio-compounds namely: Cyclo-(Leu-Pro) (1), Cyclo-(Val-Pro) (2), Cyclo-(Phe-Pro) (3), nonactin (4), monactin (5), dinactin (6) and trinactin (7). The chemical structures of compounds 1-7 were confirmed by nuclear magnetic resonance (NMR) 1D and 2D spectroscopy, mass spectrometry, and comparison with literature data. The three purified diketopiperazine (DKP) derivatives (1-3), demonstrated significant antibacterial activity against A. tumefaciens ATCC 23308 and S. typhimurium ATCC 14028. The four pure macrotetrolides (4-7), exhibited strong inhibitory effect against all tested Gram positive and Gram negative bacteria notably against A. tumefaciens ATCC 23308 and S. typhimurium ATCC 14028 with a minimum inhibitory concentration (MIC) around 8 μg/mL quite similar to that of ampicillin. Thus, we propose the use of the (SSF) active extract of the S. cavourensis TN638 strain as safe biological product to control disease caused by plant pathogen A. tumefaciens. Also, the purified active molecules produced by this strain could be used in pharmaceutical field.
DOI: 10.1016/j.micpath.2020.104106
PubMed: 32109569
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Gif-sur-Yvette, cedex, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>National Center for Scientific Research, Institute of Chemistry of Natural Substances ICSN, Avenue of the Terrasse 91198, Gif-sur-Yvette, cedex</wicri:regionArea><wicri:noRegion>cedex</wicri:noRegion><wicri:noRegion>cedex</wicri:noRegion></affiliation></author><author><name sortKey="Allouche, Noureddine" sort="Allouche, Noureddine" uniqKey="Allouche N" first="Noureddine" last="Allouche">Noureddine Allouche</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Organic Chemistry, Natural Substances Team (LR17ES08), Faculty of Sciences of Sfax, University of Sfax, PB.1171, 3000, Sfax, Tunisia.</nlm:affiliation><country xml:lang="fr">Tunisie</country><wicri:regionArea>Laboratory of Organic Chemistry, Natural Substances Team (LR17ES08), Faculty of Sciences of Sfax, University of Sfax, PB.1171, 3000, 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and Biomolecules of the Center of Biotechnology of Sfax, Road of Sidi Mansour Km 6, B.P. 1177, 3018, Sfax, Tunisia; Laboratory of Organic Chemistry, Natural Substances Team (LR17ES08), Faculty of Sciences of Sfax, University of Sfax, PB.1171, 3000, Sfax</wicri:regionArea><wicri:noRegion>Sfax</wicri:noRegion></affiliation></author><author><name sortKey="Hamed, Abdelaaty" sort="Hamed, Abdelaaty" uniqKey="Hamed A" first="Abdelaaty" last="Hamed">Abdelaaty Hamed</name><affiliation wicri:level="1"><nlm:affiliation>Chemistry Department, Faculty of Science, Al-Azhar University, Nasr City, Cairo, 11884, Egypt.</nlm:affiliation><country xml:lang="fr">Égypte</country><wicri:regionArea>Chemistry Department, Faculty of Science, Al-Azhar University, Nasr City, Cairo, 11884</wicri:regionArea><wicri:noRegion>11884</wicri:noRegion></affiliation></author><author><name sortKey="Elleuch, Lobna" sort="Elleuch, Lobna" uniqKey="Elleuch L" first="Lobna" last="Elleuch">Lobna Elleuch</name><affiliation wicri:level="1"><nlm:affiliation>CRTE Technopole of Borj-Cedria, Road of Soliman, B.P. 273, 8020, Soliman, Tunisia.</nlm:affiliation><country xml:lang="fr">Tunisie</country><wicri:regionArea>CRTE Technopole of Borj-Cedria, Road of Soliman, B.P. 273, 8020, Soliman</wicri:regionArea><wicri:noRegion>Soliman</wicri:noRegion></affiliation></author><author><name sortKey="Chakchouk Mtibaa, Ahlem" sort="Chakchouk Mtibaa, Ahlem" uniqKey="Chakchouk Mtibaa A" first="Ahlem" last="Chakchouk-Mtibaa">Ahlem Chakchouk-Mtibaa</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Microorganisms and Biomolecules of the Center of Biotechnology of Sfax, Road of Sidi Mansour Km 6, B.P. 1177, 3018, Sfax, Tunisia.</nlm:affiliation><country xml:lang="fr">Tunisie</country><wicri:regionArea>Laboratory of Microorganisms and Biomolecules of the Center of Biotechnology of Sfax, Road of Sidi Mansour Km 6, B.P. 1177, 3018, Sfax</wicri:regionArea><wicri:noRegion>Sfax</wicri:noRegion></affiliation></author><author><name sortKey="Smaoui, Slim" sort="Smaoui, Slim" uniqKey="Smaoui S" first="Slim" last="Smaoui">Slim Smaoui</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Microorganisms and Biomolecules of the Center of Biotechnology of Sfax, Road of Sidi Mansour Km 6, B.P. 1177, 3018, Sfax, Tunisia.</nlm:affiliation><country xml:lang="fr">Tunisie</country><wicri:regionArea>Laboratory of Microorganisms and Biomolecules of the Center of Biotechnology of Sfax, Road of Sidi Mansour Km 6, B.P. 1177, 3018, Sfax</wicri:regionArea><wicri:noRegion>Sfax</wicri:noRegion></affiliation></author><author><name sortKey="Karray Rebai, Ines" sort="Karray Rebai, Ines" uniqKey="Karray Rebai I" first="Ines" last="Karray-Rebai">Ines Karray-Rebai</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Microorganisms and Biomolecules of the Center of Biotechnology of Sfax, Road of Sidi Mansour Km 6, B.P. 1177, 3018, Sfax, Tunisia.</nlm:affiliation><country xml:lang="fr">Tunisie</country><wicri:regionArea>Laboratory of Microorganisms and Biomolecules of the Center of Biotechnology of Sfax, Road of Sidi Mansour Km 6, B.P. 1177, 3018, Sfax</wicri:regionArea><wicri:noRegion>Sfax</wicri:noRegion></affiliation></author><author><name sortKey="Koubaa, Imed" sort="Koubaa, Imed" uniqKey="Koubaa I" first="Imed" last="Koubaa">Imed Koubaa</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Organic Chemistry, Natural Substances Team (LR17ES08), Faculty of Sciences of Sfax, University of Sfax, PB.1171, 3000, Sfax, Tunisia.</nlm:affiliation><country xml:lang="fr">Tunisie</country><wicri:regionArea>Laboratory of Organic Chemistry, Natural Substances Team (LR17ES08), Faculty of Sciences of Sfax, University of Sfax, PB.1171, 3000, Sfax</wicri:regionArea><wicri:noRegion>Sfax</wicri:noRegion></affiliation></author><author><name sortKey="Arcile, Guillaume" sort="Arcile, Guillaume" uniqKey="Arcile G" first="Guillaume" last="Arcile">Guillaume Arcile</name><affiliation wicri:level="1"><nlm:affiliation>National Center for Scientific Research, Institute of Chemistry of Natural Substances ICSN, Avenue of the Terrasse 91198, Gif-sur-Yvette, cedex, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>National Center for Scientific Research, Institute of Chemistry of Natural Substances ICSN, Avenue of the Terrasse 91198, Gif-sur-Yvette, cedex</wicri:regionArea><wicri:noRegion>cedex</wicri:noRegion><wicri:noRegion>cedex</wicri:noRegion></affiliation></author><author><name sortKey="Allouche, Noureddine" sort="Allouche, Noureddine" uniqKey="Allouche N" first="Noureddine" last="Allouche">Noureddine Allouche</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Organic Chemistry, Natural Substances Team (LR17ES08), Faculty of Sciences of Sfax, University of Sfax, PB.1171, 3000, Sfax, Tunisia.</nlm:affiliation><country xml:lang="fr">Tunisie</country><wicri:regionArea>Laboratory of Organic Chemistry, Natural Substances Team (LR17ES08), Faculty of Sciences of Sfax, University of Sfax, PB.1171, 3000, Sfax</wicri:regionArea><wicri:noRegion>Sfax</wicri:noRegion></affiliation></author><author><name sortKey="Mellouli, Lotfi" sort="Mellouli, Lotfi" uniqKey="Mellouli L" first="Lotfi" last="Mellouli">Lotfi Mellouli</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Microorganisms and Biomolecules of the Center of Biotechnology of Sfax, Road of Sidi Mansour Km 6, B.P. 1177, 3018, Sfax, Tunisia. Electronic address: lotfi.mallouli@cbs.rnrt.tn.</nlm:affiliation><country xml:lang="fr">Tunisie</country><wicri:regionArea>Laboratory of Microorganisms and Biomolecules of the Center of Biotechnology of Sfax, Road of Sidi Mansour Km 6, B.P. 1177, 3018, Sfax</wicri:regionArea><wicri:noRegion>Sfax</wicri:noRegion></affiliation></author></analytic><series><title level="j">Microbial pathogenesis</title><idno type="eISSN">1096-1208</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The strain TN638 was isolated from Tunisian soil contaminated with industrial wastewater and selected for its potent antimicrobial activity against the tested Gram positive bacteria: Staphylococcus aureus (S. aureus) ATCC 6538 and Listeria monocytogenes (L. monocytogenes) ATCCC 19117, and Gram negative bacteria: Agrobacterium tumefaciens (A. tumefaciens) ATCC 23308 and Salmonella typhimurium (S. typhimurium) ATCC 14028 and fungi: Candida albicans (C. albicans) ATCC 10231, Rhizoctonia solani (R. solani) ATCC 58938 and Fusarium sp. Solide-state fermentation (SSF) dry crude extract of the TN638 strain presents a strong inhibitory activity notably against the phytopathogenic microorganism A. tumefaciens ATCC 23308 and the two pathogenic bacteria S. aureus ATCC 6538 and L. monocytogenes ATCCC 19117 with a zone of inhibition of 48, 34 and 34 mm respectively. According to the morphological characteristic, the complete 16S rRNA gene nucleotide sequence determination [1492 bp deposited in National Center of Biotechnology Information (NCBI) database under the accession no. LN854629.1; https://www.ncbi.nlm.nih.gov/nuccore/LN854629.1/], and the phylogenetic analysis, we can deduce that our isolate is an actinomycete bacterium belonging to the genus Streptomyces and the most closely related strain was Streptomyces cavourensis (S. cavourensis) NRRL 2740<sup>T</sup> (99.9%). We propose the assignment of our strain as Streptomyces cavourensis (S. cavourensis) TN638 strain. Work-up and purification of the strain extract using different chromatographic techniques afforded seven bio-compounds namely: Cyclo-(Leu-Pro) (1), Cyclo-(Val-Pro) (2), Cyclo-(Phe-Pro) (3), nonactin (4), monactin (5), dinactin (6) and trinactin (7). The chemical structures of compounds 1-7 were confirmed by nuclear magnetic resonance (NMR) 1D and 2D spectroscopy, mass spectrometry, and comparison with literature data. The three purified diketopiperazine (DKP) derivatives (1-3), demonstrated significant antibacterial activity against A. tumefaciens ATCC 23308 and S. typhimurium ATCC 14028. The four pure macrotetrolides (4-7), exhibited strong inhibitory effect against all tested Gram positive and Gram negative bacteria notably against A. tumefaciens ATCC 23308 and S. typhimurium ATCC 14028 with a minimum inhibitory concentration (MIC) around 8 μg/mL quite similar to that of ampicillin. Thus, we propose the use of the (SSF) active extract of the S. cavourensis TN638 strain as safe biological product to control disease caused by plant pathogen A. tumefaciens. Also, the purified active molecules produced by this strain could be used in pharmaceutical field.</div></front></TEI><affiliations><list><country><li>France</li><li>Tunisie</li><li>Égypte</li></country></list><tree><country name="Tunisie"><noRegion><name sortKey="Kaaniche, Fatma" sort="Kaaniche, Fatma" uniqKey="Kaaniche F" first="Fatma" last="Kaaniche">Fatma Kaaniche</name></noRegion><name sortKey="Allouche, Noureddine" sort="Allouche, Noureddine" uniqKey="Allouche N" first="Noureddine" last="Allouche">Noureddine Allouche</name><name sortKey="Chakchouk Mtibaa, Ahlem" sort="Chakchouk Mtibaa, Ahlem" uniqKey="Chakchouk Mtibaa A" first="Ahlem" last="Chakchouk-Mtibaa">Ahlem Chakchouk-Mtibaa</name><name sortKey="Elleuch, Lobna" sort="Elleuch, Lobna" uniqKey="Elleuch L" first="Lobna" last="Elleuch">Lobna Elleuch</name><name sortKey="Karray Rebai, Ines" sort="Karray Rebai, Ines" uniqKey="Karray Rebai I" first="Ines" last="Karray-Rebai">Ines Karray-Rebai</name><name sortKey="Koubaa, Imed" sort="Koubaa, Imed" uniqKey="Koubaa I" first="Imed" last="Koubaa">Imed Koubaa</name><name sortKey="Mellouli, Lotfi" sort="Mellouli, Lotfi" uniqKey="Mellouli L" first="Lotfi" last="Mellouli">Lotfi Mellouli</name><name sortKey="Smaoui, Slim" sort="Smaoui, Slim" uniqKey="Smaoui S" first="Slim" last="Smaoui">Slim Smaoui</name></country><country name="Égypte"><noRegion><name sortKey="Hamed, Abdelaaty" sort="Hamed, Abdelaaty" uniqKey="Hamed A" first="Abdelaaty" last="Hamed">Abdelaaty Hamed</name></noRegion></country><country name="France"><noRegion><name sortKey="Arcile, Guillaume" sort="Arcile, Guillaume" uniqKey="Arcile G" first="Guillaume" last="Arcile">Guillaume Arcile</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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